A Brief Summary of Sperm Selection Techniques and Current Approaches
Özet
The development of assisted reproductive techniques (ART), particularly intracytoplasmic sperm injection (ICSI), is crucial due to widespread infertility. Traditional sperm selection methods like swim-up and density gradient centrifugation focus on motility and morphology but often cause mechanical stress, reactive oxygen species production, and DNA fragmentation. To address these limitations, advanced techniques have emerged by mimicking natural selection or utilizing sperm membrane properties. Microfluidic systems minimize mechanical stress and simulate the female reproductive tract, improving motility and morphology. Methods utilizing electrical charge, such as the Zeta test and electrophoresis, select mature sperm with higher negative charges and intact chromatin. Apoptotic sperm can be eliminated using Annexin V and magnetic-activated cell sorting (MACS), which detects phosphatidylserine externalization. Furthermore, physiological ICSI (PICSI) leverages hyaluronic acid binding, while intracytoplasmic morphologically selected sperm injection (IMSI) allows high-magnification visual assessment of ultrastructure. Future non-invasive approaches include Raman microspectrophotometry, interferometric phase microscopy, and artificial intelligence for automated selection. While each technique has distinct advantages and limitations, mimicking natural physiological processes and developing patient-specific isolation strategies remain critical to optimizing genomic integrity, fertilization, embryo development, and overall pregnancy rates in ART.
Referanslar
Williams M, Thompson LA, Li TC, Mackenna A, Barratt CL, Cooke ID. Uterine flushing: a method to recover spermatozoa and leukocytes. Hum Reprod. 1993;8(6):925-8. doi: 10.1093/oxfordjournals.humrep.a138168. PubMed PMID: 8345087.
Ramos L, De Boer P, Meuleman EJ, Braat DD, Wetzels AM. Evaluation of ICSI-selected epididymal sperm samples of obstructive azoospermic males by the CKIA system. J Androl. 2004;25(3):406-11. doi: 10.1002/j.1939-4640.2004.tb02807.x. PubMed PMID: 15064319.
Suarez SS, Pacey AA. Sperm transport in the female reproductive tract. Hum Reprod Update. 2006;12(1):23-37. Epub 20051104. doi: 10.1093/humupd/dmi047. PubMed PMID: 16272225.
Harper JC, Robinson F, Duffy S, Griffin DK, Handyside AH, Delhanty JD, et al. Detection of fertilization in embryos with accelerated cleavage by fluorescent in-situ hybridization (FISH). Hum Reprod. 1994;9(9):1733-7. doi: 10.1093/oxfordjournals.humrep.a138784. PubMed PMID: 7836528.
Puga Molina LC, Luque GM, Balestrini PA, Marin-Briggiler CI, Romarowski A, Buffone MG. Molecular Basis of Human Sperm Capacitation. Front Cell Dev Biol. 2018;6:72. Epub 20180727. doi: 10.3389/fcell.2018.00072. PubMed PMID: 30105226; PubMed Central PMCID: PMC6078053.
Lopata A, Patullo MJ, Chang A, James B. A method for collecting motile spermatozoa from human semen. Fertil Steril. 1976;27(6):677-84. doi: 10.1016/s0015-0282(16)41899-6. PubMed PMID: 1278462.
WHO. WHO laboratory manual for the examination and processing of human semen. Kadıoğlu A, editor. İstanbul: Nobel Tıp Kitabevleri; 2021.
Mahadevan M, Baker G. Assessment and Preparation of Semen for in Vitro Fertilization Clinical in Vitro Fertilization. In: Trounson A, editor. London, UK: Springer; 1984. p. 83-97.
Harrison RA. A highly efficient method for washing mammalian spermatozoa. J Reprod Fertil. 1976;48(2):347-53. doi: 10.1530/jrf.0.0480347. PubMed PMID: 994106.
Baldini D, Ferri D, Baldini GM, Lot D, Catino A, Vizziello D, et al. Sperm Selection for ICSI: Do We Have a Winner? Cells. 2021;10(12). Epub 20211217. doi: 10.3390/cells10123566. PubMed PMID: 34944074; PubMed Central PMCID: PMC8700516.
Natali I. Sperm Preparation Techniques for Artificial Insemination - Comparison of Sperm Washing, Swim Up, and Density Gradient Centrifugation Methods. In: Milad M, editor. Artificial Insemination in Farm Animals: IntechOpen; 2011.
Muratori M, Tarozzi N, Carpentiero F, Danti S, Perrone FM, Cambi M, et al. Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa. Sci Rep. 2019;9(1):7492. Epub 20190516. doi: 10.1038/s41598-019-43981-2. PubMed PMID: 31097741; PubMed Central PMCID: PMC6522556.
Reece A, Xia B, Jiang Z, Noren B, McBride R, Oakey J. Microfluidic techniques for high throughput single cell analysis. Curr Opin Biotechnol. 2016;40:90-6. Epub 20160328. doi: 10.1016/j.copbio.2016.02.015. PubMed PMID: 27032065; PubMed Central PMCID: PMC4975615.
Song YS, Moon S, Hulli L, Hasan SK, Kayaalp E, Demirci U. Microfluidics for cryopreservation. Lab Chip. 2009;9(13):1874-81. Epub 20090331. doi: 10.1039/b823062e. PubMed PMID: 19532962; PubMed Central PMCID: PMC2719835.
Suh R, Takayama S, Smith GD. Microfluidic applications for andrology. J Androl. 2005;26(6):664-70. doi: 10.2164/jandrol.05119. PubMed PMID: 16291957.
Schuster TG, Cho B, Keller LM, Takayama S, Smith GD. Isolation of motile spermatozoa from semen samples using microfluidics. Reprod Biomed Online. 2003;7(1):75-81. doi: 10.1016/s1472-6483(10)61732-4. PubMed PMID: 12930579.
Shirota K, Yotsumoto F, Itoh H, Obama H, Hidaka N, Nakajima K, et al. Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage. Fertil Steril. 2016;105(2):315-21 e1. Epub 20151106. doi: 10.1016/j.fertnstert.2015.10.023. PubMed PMID: 26551440.
Ma R, Xie L, Han C, Su K, Qiu T, Wang L, et al. In vitro fertilization on a single-oocyte positioning system integrated with motile sperm selection and early embryo development. Anal Chem. 2011;83(8):2964-70. Epub 20110323. doi: 10.1021/ac103063g. PubMed PMID: 21438638.
Ainsworth C, Nixon B, Aitken RJ. Development of a novel electrophoretic system for the isolation of human spermatozoa. Hum Reprod. 2005;20(8):2261-70. Epub 20050414. doi: 10.1093/humrep/dei024. PubMed PMID: 15831507.
Smith GD, Takayama S. Application of microfluidic technologies to human assisted reproduction. Mol Hum Reprod. 2017;23(4):257-68. doi: 10.1093/molehr/gaw076. PubMed PMID: 28130394; PubMed Central PMCID: PMC5387843.
Asghar W, Velasco V, Kingsley JL, Shoukat MS, Shafiee H, Anchan RM, et al. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater. 2014;3(10):1671-9. Epub 20140417. doi: 10.1002/adhm.201400058. PubMed PMID: 24753434; PubMed Central PMCID: PMC4194169.
Smith GD, Takayama S, Swain JE. Rethinking in vitro embryo culture: new developments in culture platforms and potential to improve assisted reproductive technologies. Biol Reprod. 2012;86(3):62. Epub 20120308. doi: 10.1095/biolreprod.111.095778. PubMed PMID: 21998170; PubMed Central PMCID: PMC3316262.
Parrella A, Choi D, Keating D, Rosenwaks Z, Palermo GD. A Microfluidic Device for Selecting the Most Progressively Motile Spermatozoa Yields a Higher Rate of Euploid Embryos. Fertility and Sterility. 2018;110(4):E342-E. PubMed PMID: WOS:000448713601270.
Samuel R, Feng H, Jafek A, Despain D, Jenkins T, Gale B. Microfluidic-based sperm sorting & analysis for treatment of male infertility. Transl Androl Urol. 2018;7(Suppl 3):S336-S47. doi: 10.21037/tau.2018.05.08. PubMed PMID: 30159240; PubMed Central PMCID: PMC6087839.
Ishijima SA, Okuno M, Mohri H. Zeta potential of human X- and Y-bearing sperm. Int J Androl. 1991;14(5):340-7. doi: 10.1111/j.1365-2605.1991.tb01102.x. PubMed PMID: 1794918.
Kirchhoff C, Schroter S. New insights into the origin, structure and role of CD52: a major component of the mammalian sperm glycocalyx. Cells Tissues Organs. 2001;168(1-2):93-104. doi: 10.1159/000016810. PubMed PMID: 11114591.
Ainsworth CJ, Nixon B, Aitken RJ. The electrophoretic separation of spermatozoa: an analysis of genotype, surface carbohydrate composition and potential for capacitation. Int J Androl. 2011;34(5 Pt 2):e422-34. Epub 20110512. doi: 10.1111/j.1365-2605.2011.01164.x. PubMed PMID: 21564134.
Aitken RJ, Hanson AR, Kuczera L. Electrophoretic sperm isolation: optimization of electrophoresis conditions and impact on oxidative stress. Hum Reprod. 2011;26(8):1955-64. Epub 20110610. doi: 10.1093/humrep/der162. PubMed PMID: 21665871.
Giuliani V, Pandolfi C, Santucci R, Pelliccione F, Macerola B, Focarelli R, et al. Expression of gp20, a human sperm antigen of epididymal origin, is reduced in spermatozoa from subfertile men. Mol Reprod Dev. 2004;69(2):235-40. doi: 10.1002/mrd.20166. PubMed PMID: 15293226.
Chaudhuri AR, Datta H. A novel technique for isolation of pure sperm heads from disintegrated mammalian spermatozoa. Prep Biochem. 1994;24(3-4):185-92. doi: 10.1080/10826069408010092. PubMed PMID: 7831201.
Engelmann U, Krassnigg F, Schatz H, Schill WB. Separation of human X and Y spermatozoa by free-flow electrophoresis. Gamete Res. 1988;19(2):151-60. doi: 10.1002/mrd.1120190205. PubMed PMID: 3209178.
Chan PJ, Jacobson JD, Corselli JU, Patton WC. A simple zeta method for sperm selection based on membrane charge. Fertil Steril. 2006;85(2):481-6. doi: 10.1016/j.fertnstert.2005.07.1302. PubMed PMID: 16595231.
Kam TL, Jacobson JD, Patton WC, Corselli JU, Chan PJ. Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection. J Assist Reprod Genet. 2007;24(9):429-34. Epub 20070726. doi: 10.1007/s10815-007-9158-1. PubMed PMID: 17653847; PubMed Central PMCID: PMC3454951.
Esfahani MHN, Razavi S, Mardani M, Khajavi NA, Deemeh MR, Tavalaee M. The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure. Yakhteh. 2009;11(2):168-75. PubMed PMID: WOS:000207859100009.
Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, et al. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod. 2009;24(10):2409-16. Epub 20090624. doi: 10.1093/humrep/dep088. PubMed PMID: 19553239.
Simon L, Ge SQ, Carrell DT. Sperm selection based on electrostatic charge. Methods Mol Biol. 2013;927:269-78. doi: 10.1007/978-1-62703-038-0_25. PubMed PMID: 22992922.
Simon L, Murphy K, Aston KI, Emery BR, Hotaling JM, Carrell DT. Micro-electrophoresis: a noninvasive method of sperm selection based on membrane charge. Fertil Steril. 2015;103(2):361-6 e3. Epub 20141212. doi: 10.1016/j.fertnstert.2014.10.047. PubMed PMID: 25500020.
Simon L, Murphy K, Aston KI, Emery BR, Hotaling JM, Carrell DT. Optimization of microelectrophoresis to select highly negatively charged sperm. J Assist Reprod Genet. 2016;33(6):679-88. Epub 20160323. doi: 10.1007/s10815-016-0700-x. PubMed PMID: 27007874; PubMed Central PMCID: PMC4889482.
van Heerde WL, de Groot PG, Reutelingsperger CP. The complexity of the phospholipid binding protein Annexin V. Thromb Haemost. 1995;73(2):172-9. PubMed PMID: 7792726.
Glander HJ, Schaller J. Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. Mol Hum Reprod. 1999;5(2):109-15. doi: 10.1093/molehr/5.2.109. PubMed PMID: 10065865.
Sakkas D, Mariethoz E, St John JC. Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway. Exp Cell Res. 1999;251(2):350-5. doi: 10.1006/excr.1999.4586. PubMed PMID: 10471320.
Grunewald S, Paasch U, Glander HJ. Enrichment of non-apoptotic human spermatozoa after cryopreservation by immunomagnetic cell sorting. Cell Tissue Bank. 2001;2(3):127-33. doi: 10.1023/A:1020188913551. PubMed PMID: 15256910.
de Vantery Arrighi C, Lucas H, Chardonnens D, de Agostini A. Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol. 2009;7:1. Epub 20090108. doi: 10.1186/1477-7827-7-1. PubMed PMID: 19133142; PubMed Central PMCID: PMC2636817.
Said TM, Grunewald S, Paasch U, Glander HJ, Baumann T, Kriegel C, et al. Advantage of combining magnetic cell separation with sperm preparation techniques. Reprod Biomed Online. 2005;10(6):740-6. doi: 10.1016/s1472-6483(10)61118-2. PubMed PMID: 15970004.
Chung MK, Chiu PC, Lee CL, Pang RT, Ng EH, Lee KF, et al. Cumulus-associated alpha2-macroglobulin derivative retains proconceptive glycodelin-C in the human cumulus matrix. Hum Reprod. 2009;24(11):2856-67. Epub 20090722. doi: 10.1093/humrep/dep265. PubMed PMID: 19625311.
Cayli S, Jakab A, Ovari L, Delpiano E, Celik-Ozenci C, Sakkas D, et al. Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online. 2003;7(4):462-8. doi: 10.1016/s1472-6483(10)61891-3. PubMed PMID: 14656409.
Huszar G, Ozkavukcu S, Jakab A, Celik-Ozenci C, Sati GL, Cayli S. Hyaluronic acid binding ability of human sperm reflects cellular maturity and fertilizing potential: selection of sperm for intracytoplasmic sperm injection. Curr Opin Obstet Gynecol. 2006;18(3):260-7. doi: 10.1097/01.gco.0000193018.98061.2f. PubMed PMID: 16735824.
Barak Y, Menezo Y, Veiga A, Elder K. A physiological replacement for polyvinylpyrrolidone (PVP) in assisted reproductive technology. Hum Fertil (Camb). 2001;4(2):99-103. doi: 10.1080/1464727012000199371. PubMed PMID: 11591264.
Parmegiani L, Cognigni GE, Ciampaglia W, Pocognoli P, Marchi F, Filicori M. Efficiency of hyaluronic acid (HA) sperm selection. J Assist Reprod Genet. 2010;27(1):13-6. Epub 20091230. doi: 10.1007/s10815-009-9380-0. PubMed PMID: 20041286; PubMed Central PMCID: PMC2826621.
Ye H, Huang GN, Gao Y, Liu DY. Relationship between human sperm-hyaluronan binding assay and fertilization rate in conventional in vitro fertilization. Hum Reprod. 2006;21(6):1545-50. Epub 20060404. doi: 10.1093/humrep/del008. PubMed PMID: 16595551.
Bartoov B, Berkovitz A, Eltes F. Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med. 2001;345(14):1067-8. doi: 10.1056/NEJM200110043451416. PubMed PMID: 11586970.
Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, et al. Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril. 2003;80(6):1413-9. doi: 10.1016/j.fertnstert.2003.05.016. PubMed PMID: 14667877.
Franco JG, Jr., Baruffi RL, Mauri AL, Petersen CG, Oliveira JB, Vagnini L. Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod Biomed Online. 2008;17(1):42-5. doi: 10.1016/s1472-6483(10)60291-x. PubMed PMID: 18616888.
Hazout A, Dumont-Hassan M, Junca AM, Cohen Bacrie P, Tesarik J. High-magnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online. 2006;12(1):19-25. doi: 10.1016/s1472-6483(10)60975-3. PubMed PMID: 16454928.
Teixeira DM, Hadyme Miyague A, Barbosa MA, Navarro PA, Raine-Fenning N, Nastri CO, et al. Regular (ICSI) versus ultra-high magnification (IMSI) sperm selection for assisted reproduction. Cochrane Database Syst Rev. 2020;2:CD010167. Epub 20200221. doi: 10.1002/14651858.CD010167.pub3. PubMed PMID: 32083321; PubMed Central PMCID: PMC7033651.
Fortunato A, Boni R, Leo R, Nacchia G, Liguori F, Casale S, et al. Vacuoles in sperm head are not associated with head morphology, DNA damage and reproductive success. Reprod Biomed Online. 2016;32(2):154-61. Epub 20151110. doi: 10.1016/j.rbmo.2015.10.009. PubMed PMID: 26655650.
Huser T, Orme CA, Hollars CW, Corzett MH, Balhorn R. Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells. J Biophotonics. 2009;2(5):322-32. doi: 10.1002/jbio.200910012. PubMed PMID: 19373853.
Itzkan I, Qiu L, Fang H, Zaman MM, Vitkin E, Ghiran IC, et al. Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels. Proc Natl Acad Sci U S A. 2007;104(44):17255-60. Epub 20071023. doi: 10.1073/pnas.0708669104. PubMed PMID: 17956980; PubMed Central PMCID: PMC2077242.
Ghasemian F, Mirroshandel SA, Monji-Azad S, Azarnia M, Zahiri Z. An efficient method for automatic morphological abnormality detection from human sperm images. Comput Methods Programs Biomed. 2015;122(3):409-20. Epub 20150904. doi: 10.1016/j.cmpb.2015.08.013. PubMed PMID: 26345335.
Mirsky SK, Barnea I, Levi M, Greenspan H, Shaked NT. Automated analysis of individual sperm cells using stain-free interferometric phase microscopy and machine learning. Cytometry A. 2017;91(9):893-900. Epub 20170822. doi: 10.1002/cyto.a.23189. PubMed PMID: 28834185.
McCallum C, Riordon J, Wang Y, Kong T, You JB, Sanner S, et al. Deep learning-based selection of human sperm with high DNA integrity. Commun Biol. 2019;2:250. Epub 20190703. doi: 10.1038/s42003-019-0491-6. PubMed PMID: 31286067; PubMed Central PMCID: PMC6610103.
