İmmünokimyasal Yöntemlerde Biyotin İnterferansı
Özet
Güncel Biyokimya Çalışmaları II eserinde yer alan bu bölümde, Uzm. Dr. Emiş Deniz Akbulut tarafından hazırlanan "İmmünokimyasal Yöntemlerde Biyotin İnterferansı" başlıklı çalışma, biyotin (B7 vitamini) kullanımının klinik laboratuvar testleri üzerindeki olumsuz etkilerini ve analitik interferans mekanizmalarını ayrıntılı olarak incelemektedir. Karbonhidrat, lipit ve amino asit metabolizmasında hayati koenzim görevi üstlenen biyotin; günümüzde kozmetik takviyelerin yanı sıra biyotinidaz eksikliği ve multipl skleroz gibi doğumsal veya nörolojik hastalıkların tedavisinde yüksek ve mega dozlarda yaygın biçimde tercih edilmektedir. Biyotin ile streptavidin arasındaki güçlü biyokimyasal etkileşim immünokimyasal test tasarımlarında sıkça kullanılsa da, hasta serumundaki yüksek biyotin konsantrasyonu ayırma basamağını bozarak sandviç yöntemlerde yalancı düşük, kompetitif yöntemlerde ise yalancı yüksek sonuçlara yol açmaktadır. Bu durum, hipertiroidizm veya D vitamini toksikasyonu gibi endokrin patolojileri taklit eden tutarsız laboratuvar profillerine ve dolayısıyla yanlış klinik teşhislere zemin hazırlayabilmektedir. Bölüm; hekim, hasta, laboratuvar ve üretici firmalar arasındaki iletişimin artırılması, detaylı hasta anamnezinin sorgulanması, biyotinle etkileşime girmeyen alternatif yöntemlerin değerlendirilmesi ve analitik farkındalık oluşturulmasının doğru tanı ve hasta güvenliği açısından taşıdığı hayati önemi ortaya koymaktadır.
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